Proteins bind to ring to repair DNA

Researchers have used crystallography techniques to image how the non-homologous end-joining (NHEJ) proteins APLF (A-KBM) and XLF (X-KBM) bind to a ring-shaped protein Ku70–Ku80 (Ku) complexed to DNA. The work could allow us to better understand how this “molecular ballet” helps cells repair their DNA and shed more light on why certain cancer cells are resistant to radiotherapy. The team includes scientists from: the Institute for Integrative Biology of the CellInstitute Joliot, CEA, CNRS, Université Paris-Sud, Université Paris-SaclayInstitut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS; Equipe Labellisée Ligue Contre le Cancer 2018, Toulouse; Synchrotron SoleilSignalisations, Noyaux et Innovations en Cancérologie, UMR 8126; and the Cancer Research Center of Marseille, CNRS UMR 7258, Inserm U1068, Institut Paoli-Calmettes, Aix-Marseille Université UM105, Marseille.

Reference: XLF and APLF bind to Ku80 on two remote sites to ensure repair by non-homologous end-joining, Clement Nemoz et. al., Nature Structural & Molecular Biology 10.1038/s41594-018-0133-6

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A candidate molecule for regenerating the brain

The adult brain only has a very limited capacity to regenerate. Researchers at the Institut de Radiobiologie Cellulaire et Moléculaire (IRCM***) have now shown, however, that the Syndecan-1 molecule, which is present in the membrane of neural stem cells, plays an important role in the proliferation of these cells in the adult mouse brain. Syndecan-1 could thus be used as a possible tracer to track regeneration in the damaged brain and help in the development of therapeutic strategies. The research is detailed in Stem Cell Reports.

*** UMR967, Institut François Jacob, CEA Paris Saclay ; Inserm ; Université Paris-Sud / Paris Saclay ; Université Paris Diderot.

Reference: Distinct Molecular Signatures of Quiescent and Activated Adult Neural Stem Cells Reveal Specific Interactions with Their Microenvironment, Lise Morizur et. al., Stem Cell Reports, 14.8.2018.

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