Researchers have used crystallography techniques to image how the non-homologous end-joining (NHEJ) proteins APLF (A-KBM) and XLF (X-KBM) bind to a ring-shaped protein Ku70–Ku80 (Ku) complexed to DNA. The work could allow us to better understand how this “molecular ballet” helps cells repair their DNA and shed more light on why certain cancer cells are resistant to radiotherapy. The team includes scientists from: the Institute for Integrative Biology of the Cell, Institute Joliot, CEA, CNRS, Université Paris-Sud, Université Paris-Saclay; Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS; Equipe Labellisée Ligue Contre le Cancer 2018, Toulouse; Synchrotron Soleil; Signalisations, Noyaux et Innovations en Cancérologie, UMR 8126; and the Cancer Research Center of Marseille, CNRS UMR 7258, Inserm U1068, Institut Paoli-Calmettes, Aix-Marseille Université UM105, Marseille.
Reference: XLF and APLF bind to Ku80 on two remote sites to ensure repair by non-homologous end-joining, Clement Nemoz et. al., Nature Structural & Molecular Biology 10.1038/s41594-018-0133-6